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pbabe puro empty vector control  (Addgene inc)


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    Structured Review

    Addgene inc pbabe puro empty vector control
    Pbabe Puro Empty Vector Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 581 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+puro+empty+vector+control/pBABE-puro+(Plasmid+%231764)/10__1158_slash_2767___9764__crc___23___0379-57-43-47
    Average 96 stars, based on 581 article reviews
    pbabe puro empty vector control - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: PP2A-activating Drugs Enhance FLT3 Inhibitor Efficacy through AKT Inhibition–Dependent GSK-3β–Mediated c-Myc and Pim-1 Proteasomal Degradation
    Article Snippet: .. The myc-estrogen receptor (ER) expression vector pBABE-puromyc-ER (plasmid No. 19128; ref. 21) and pBABE-puro empty vector control were from Addgene. .. Approximately 80% confluent PhoenixAMPHO packaging cells (ATCC CRL-3213) were incubated in 25 mmol/L chloroquine for 1 hour and then transfected with 20-mg retroviral plasmidDNAby the calciumphosphatemethod.

    Article Title: Pim kinase inhibitor co-treatment decreases alternative non-homologous end-joining DNA repair and genomic instability induced by topoisomerase 2 inhibitors in cells with FLT3 internal tandem duplication
    Article Snippet: Ba/F3-ITD cells were infected with pMSCVpuro-Flag-cMyc-T58A retroviral plasmid (Addgene plasmid #20076; https://www.addgene.org/20076 ) [ ]) from Addgene (Cambridge, MA, USA), containing c-Myc with a point mutation at position 58, changing threonine to alanine and thereby inhibiting phosphorylation, or pMSCVpuro empty vector control (Takara Bio USA, Mountain View, CA, USA). .. To induce c-Myc overexpression, Ba/F3-ITD cells were infected with the c-Myc expression vector pBABEpuro-myc-ER (estrogen receptor) (plasmid #19128; https://www.addgene.org/19128 ) [ ] or pBABE-puro empty vector control from Addgene. ..

    Plasmid Preparation:

    Article Title: PP2A-activating Drugs Enhance FLT3 Inhibitor Efficacy through AKT Inhibition–Dependent GSK-3β–Mediated c-Myc and Pim-1 Proteasomal Degradation
    Article Snippet: .. The myc-estrogen receptor (ER) expression vector pBABE-puromyc-ER (plasmid No. 19128; ref. 21) and pBABE-puro empty vector control were from Addgene. .. Approximately 80% confluent PhoenixAMPHO packaging cells (ATCC CRL-3213) were incubated in 25 mmol/L chloroquine for 1 hour and then transfected with 20-mg retroviral plasmidDNAby the calciumphosphatemethod.

    Article Title: Pim Kinase Inhibitors Increase Gilteritinib Cytotoxicity in FLT3-ITD Acute Myeloid Leukemia Through GSK-3β Activation and c-Myc and Mcl-1 Proteasomal Degradation
    Article Snippet: .. Ba/F3-ITD cells also underwent retroviral infection with pBabe-Flag hMcl-1-S159A plasmid (Addgene plasmid # 25372; RRID:Addgene_25372 ) containing Mcl-1 with a mutation changing serine to alanine at residue 159, preventing phosphorylation , pBabe-Flag hMcl-1 plasmid (Addgene plasmid # 25371; RRID:Addgene_25371 ), containing wild-type Mcl-1 , or pBabe -puro empty vector control (Addgene plasmid # 1764; RRID:Addgene_1764 ; ref. ). .. Finally, Ba/F3-ITD cells underwent retroviral infection with pBabe-Puro-Myr-Flag-AKT1 (Addgene plasmid # 15294; RRID:Addgene_15294 ; ref. ), containing myristoylated (constitutively activated) AKT (myr-AKT), or pBABE-puro empty vector control, as described previously ( ).

    Article Title: Pim kinase inhibitors increase gilteritinib cytotoxicity in FLT3-ITD acute myeloid leukemia through GSK-3β activation and c-Myc and Mcl-1 proteasomal degradation
    Article Snippet: .. Ba/F3-ITD cells also underwent retroviral infection with pBabe-Flag hMcl-1S159A plasmid (Addgene plasmid # 25372; RRID:Addgene_25372) containing Mcl-1 with a mutation changing serine to alanine at residue 159, preventing phosphorylation (27), pBabe-Flag hMcl-1 plasmid (Addgene plasmid # 25371; RRID:Addgene_25371), containing wild-type Mcl-1 (27), or pBabe-puro empty vector control (Addgene plasmid # 1764; RRID:Addgene_1764; ref. 24). .. Finally, Ba/F3-ITD cells underwent retroviral infection with pBabe-PuroMyr-Flag-AKT1 (Addgene plasmid # 15294; RRID:Addgene_15294; ref. 28), containing myristoylated (constitutively activated) AKT (myr-AKT), or pBABE-puro empty vector control, as described previously (24).

    Article Title: Pim kinase inhibitor co-treatment decreases alternative non-homologous end-joining DNA repair and genomic instability induced by topoisomerase 2 inhibitors in cells with FLT3 internal tandem duplication
    Article Snippet: Ba/F3-ITD cells were infected with pMSCVpuro-Flag-cMyc-T58A retroviral plasmid (Addgene plasmid #20076; https://www.addgene.org/20076 ) [ ]) from Addgene (Cambridge, MA, USA), containing c-Myc with a point mutation at position 58, changing threonine to alanine and thereby inhibiting phosphorylation, or pMSCVpuro empty vector control (Takara Bio USA, Mountain View, CA, USA). .. To induce c-Myc overexpression, Ba/F3-ITD cells were infected with the c-Myc expression vector pBABEpuro-myc-ER (estrogen receptor) (plasmid #19128; https://www.addgene.org/19128 ) [ ] or pBABE-puro empty vector control from Addgene. ..

    Control:

    Article Title: PP2A-activating Drugs Enhance FLT3 Inhibitor Efficacy through AKT Inhibition–Dependent GSK-3β–Mediated c-Myc and Pim-1 Proteasomal Degradation
    Article Snippet: .. The myc-estrogen receptor (ER) expression vector pBABE-puromyc-ER (plasmid No. 19128; ref. 21) and pBABE-puro empty vector control were from Addgene. .. Approximately 80% confluent PhoenixAMPHO packaging cells (ATCC CRL-3213) were incubated in 25 mmol/L chloroquine for 1 hour and then transfected with 20-mg retroviral plasmidDNAby the calciumphosphatemethod.

    Article Title: Pim Kinase Inhibitors Increase Gilteritinib Cytotoxicity in FLT3-ITD Acute Myeloid Leukemia Through GSK-3β Activation and c-Myc and Mcl-1 Proteasomal Degradation
    Article Snippet: .. Ba/F3-ITD cells also underwent retroviral infection with pBabe-Flag hMcl-1-S159A plasmid (Addgene plasmid # 25372; RRID:Addgene_25372 ) containing Mcl-1 with a mutation changing serine to alanine at residue 159, preventing phosphorylation , pBabe-Flag hMcl-1 plasmid (Addgene plasmid # 25371; RRID:Addgene_25371 ), containing wild-type Mcl-1 , or pBabe -puro empty vector control (Addgene plasmid # 1764; RRID:Addgene_1764 ; ref. ). .. Finally, Ba/F3-ITD cells underwent retroviral infection with pBabe-Puro-Myr-Flag-AKT1 (Addgene plasmid # 15294; RRID:Addgene_15294 ; ref. ), containing myristoylated (constitutively activated) AKT (myr-AKT), or pBABE-puro empty vector control, as described previously ( ).

    Article Title: Pim kinase inhibitors increase gilteritinib cytotoxicity in FLT3-ITD acute myeloid leukemia through GSK-3β activation and c-Myc and Mcl-1 proteasomal degradation
    Article Snippet: .. Ba/F3-ITD cells also underwent retroviral infection with pBabe-Flag hMcl-1S159A plasmid (Addgene plasmid # 25372; RRID:Addgene_25372) containing Mcl-1 with a mutation changing serine to alanine at residue 159, preventing phosphorylation (27), pBabe-Flag hMcl-1 plasmid (Addgene plasmid # 25371; RRID:Addgene_25371), containing wild-type Mcl-1 (27), or pBabe-puro empty vector control (Addgene plasmid # 1764; RRID:Addgene_1764; ref. 24). .. Finally, Ba/F3-ITD cells underwent retroviral infection with pBabe-PuroMyr-Flag-AKT1 (Addgene plasmid # 15294; RRID:Addgene_15294; ref. 28), containing myristoylated (constitutively activated) AKT (myr-AKT), or pBABE-puro empty vector control, as described previously (24).

    Article Title: Pim kinase inhibitor co-treatment decreases alternative non-homologous end-joining DNA repair and genomic instability induced by topoisomerase 2 inhibitors in cells with FLT3 internal tandem duplication
    Article Snippet: Ba/F3-ITD cells were infected with pMSCVpuro-Flag-cMyc-T58A retroviral plasmid (Addgene plasmid #20076; https://www.addgene.org/20076 ) [ ]) from Addgene (Cambridge, MA, USA), containing c-Myc with a point mutation at position 58, changing threonine to alanine and thereby inhibiting phosphorylation, or pMSCVpuro empty vector control (Takara Bio USA, Mountain View, CA, USA). .. To induce c-Myc overexpression, Ba/F3-ITD cells were infected with the c-Myc expression vector pBABEpuro-myc-ER (estrogen receptor) (plasmid #19128; https://www.addgene.org/19128 ) [ ] or pBABE-puro empty vector control from Addgene. ..

    Retroviral:

    Article Title: Pim Kinase Inhibitors Increase Gilteritinib Cytotoxicity in FLT3-ITD Acute Myeloid Leukemia Through GSK-3β Activation and c-Myc and Mcl-1 Proteasomal Degradation
    Article Snippet: .. Ba/F3-ITD cells also underwent retroviral infection with pBabe-Flag hMcl-1-S159A plasmid (Addgene plasmid # 25372; RRID:Addgene_25372 ) containing Mcl-1 with a mutation changing serine to alanine at residue 159, preventing phosphorylation , pBabe-Flag hMcl-1 plasmid (Addgene plasmid # 25371; RRID:Addgene_25371 ), containing wild-type Mcl-1 , or pBabe -puro empty vector control (Addgene plasmid # 1764; RRID:Addgene_1764 ; ref. ). .. Finally, Ba/F3-ITD cells underwent retroviral infection with pBabe-Puro-Myr-Flag-AKT1 (Addgene plasmid # 15294; RRID:Addgene_15294 ; ref. ), containing myristoylated (constitutively activated) AKT (myr-AKT), or pBABE-puro empty vector control, as described previously ( ).

    Article Title: Pim kinase inhibitors increase gilteritinib cytotoxicity in FLT3-ITD acute myeloid leukemia through GSK-3β activation and c-Myc and Mcl-1 proteasomal degradation
    Article Snippet: .. Ba/F3-ITD cells also underwent retroviral infection with pBabe-Flag hMcl-1S159A plasmid (Addgene plasmid # 25372; RRID:Addgene_25372) containing Mcl-1 with a mutation changing serine to alanine at residue 159, preventing phosphorylation (27), pBabe-Flag hMcl-1 plasmid (Addgene plasmid # 25371; RRID:Addgene_25371), containing wild-type Mcl-1 (27), or pBabe-puro empty vector control (Addgene plasmid # 1764; RRID:Addgene_1764; ref. 24). .. Finally, Ba/F3-ITD cells underwent retroviral infection with pBabe-PuroMyr-Flag-AKT1 (Addgene plasmid # 15294; RRID:Addgene_15294; ref. 28), containing myristoylated (constitutively activated) AKT (myr-AKT), or pBABE-puro empty vector control, as described previously (24).

    Infection:

    Article Title: Pim Kinase Inhibitors Increase Gilteritinib Cytotoxicity in FLT3-ITD Acute Myeloid Leukemia Through GSK-3β Activation and c-Myc and Mcl-1 Proteasomal Degradation
    Article Snippet: .. Ba/F3-ITD cells also underwent retroviral infection with pBabe-Flag hMcl-1-S159A plasmid (Addgene plasmid # 25372; RRID:Addgene_25372 ) containing Mcl-1 with a mutation changing serine to alanine at residue 159, preventing phosphorylation , pBabe-Flag hMcl-1 plasmid (Addgene plasmid # 25371; RRID:Addgene_25371 ), containing wild-type Mcl-1 , or pBabe -puro empty vector control (Addgene plasmid # 1764; RRID:Addgene_1764 ; ref. ). .. Finally, Ba/F3-ITD cells underwent retroviral infection with pBabe-Puro-Myr-Flag-AKT1 (Addgene plasmid # 15294; RRID:Addgene_15294 ; ref. ), containing myristoylated (constitutively activated) AKT (myr-AKT), or pBABE-puro empty vector control, as described previously ( ).

    Article Title: Pim kinase inhibitors increase gilteritinib cytotoxicity in FLT3-ITD acute myeloid leukemia through GSK-3β activation and c-Myc and Mcl-1 proteasomal degradation
    Article Snippet: .. Ba/F3-ITD cells also underwent retroviral infection with pBabe-Flag hMcl-1S159A plasmid (Addgene plasmid # 25372; RRID:Addgene_25372) containing Mcl-1 with a mutation changing serine to alanine at residue 159, preventing phosphorylation (27), pBabe-Flag hMcl-1 plasmid (Addgene plasmid # 25371; RRID:Addgene_25371), containing wild-type Mcl-1 (27), or pBabe-puro empty vector control (Addgene plasmid # 1764; RRID:Addgene_1764; ref. 24). .. Finally, Ba/F3-ITD cells underwent retroviral infection with pBabe-PuroMyr-Flag-AKT1 (Addgene plasmid # 15294; RRID:Addgene_15294; ref. 28), containing myristoylated (constitutively activated) AKT (myr-AKT), or pBABE-puro empty vector control, as described previously (24).

    Article Title: Pim kinase inhibitor co-treatment decreases alternative non-homologous end-joining DNA repair and genomic instability induced by topoisomerase 2 inhibitors in cells with FLT3 internal tandem duplication
    Article Snippet: Ba/F3-ITD cells were infected with pMSCVpuro-Flag-cMyc-T58A retroviral plasmid (Addgene plasmid #20076; https://www.addgene.org/20076 ) [ ]) from Addgene (Cambridge, MA, USA), containing c-Myc with a point mutation at position 58, changing threonine to alanine and thereby inhibiting phosphorylation, or pMSCVpuro empty vector control (Takara Bio USA, Mountain View, CA, USA). .. To induce c-Myc overexpression, Ba/F3-ITD cells were infected with the c-Myc expression vector pBABEpuro-myc-ER (estrogen receptor) (plasmid #19128; https://www.addgene.org/19128 ) [ ] or pBABE-puro empty vector control from Addgene. ..

    Mutagenesis:

    Article Title: Pim Kinase Inhibitors Increase Gilteritinib Cytotoxicity in FLT3-ITD Acute Myeloid Leukemia Through GSK-3β Activation and c-Myc and Mcl-1 Proteasomal Degradation
    Article Snippet: .. Ba/F3-ITD cells also underwent retroviral infection with pBabe-Flag hMcl-1-S159A plasmid (Addgene plasmid # 25372; RRID:Addgene_25372 ) containing Mcl-1 with a mutation changing serine to alanine at residue 159, preventing phosphorylation , pBabe-Flag hMcl-1 plasmid (Addgene plasmid # 25371; RRID:Addgene_25371 ), containing wild-type Mcl-1 , or pBabe -puro empty vector control (Addgene plasmid # 1764; RRID:Addgene_1764 ; ref. ). .. Finally, Ba/F3-ITD cells underwent retroviral infection with pBabe-Puro-Myr-Flag-AKT1 (Addgene plasmid # 15294; RRID:Addgene_15294 ; ref. ), containing myristoylated (constitutively activated) AKT (myr-AKT), or pBABE-puro empty vector control, as described previously ( ).

    Article Title: Pim kinase inhibitors increase gilteritinib cytotoxicity in FLT3-ITD acute myeloid leukemia through GSK-3β activation and c-Myc and Mcl-1 proteasomal degradation
    Article Snippet: .. Ba/F3-ITD cells also underwent retroviral infection with pBabe-Flag hMcl-1S159A plasmid (Addgene plasmid # 25372; RRID:Addgene_25372) containing Mcl-1 with a mutation changing serine to alanine at residue 159, preventing phosphorylation (27), pBabe-Flag hMcl-1 plasmid (Addgene plasmid # 25371; RRID:Addgene_25371), containing wild-type Mcl-1 (27), or pBabe-puro empty vector control (Addgene plasmid # 1764; RRID:Addgene_1764; ref. 24). .. Finally, Ba/F3-ITD cells underwent retroviral infection with pBabe-PuroMyr-Flag-AKT1 (Addgene plasmid # 15294; RRID:Addgene_15294; ref. 28), containing myristoylated (constitutively activated) AKT (myr-AKT), or pBABE-puro empty vector control, as described previously (24).

    Residue:

    Article Title: Pim Kinase Inhibitors Increase Gilteritinib Cytotoxicity in FLT3-ITD Acute Myeloid Leukemia Through GSK-3β Activation and c-Myc and Mcl-1 Proteasomal Degradation
    Article Snippet: .. Ba/F3-ITD cells also underwent retroviral infection with pBabe-Flag hMcl-1-S159A plasmid (Addgene plasmid # 25372; RRID:Addgene_25372 ) containing Mcl-1 with a mutation changing serine to alanine at residue 159, preventing phosphorylation , pBabe-Flag hMcl-1 plasmid (Addgene plasmid # 25371; RRID:Addgene_25371 ), containing wild-type Mcl-1 , or pBabe -puro empty vector control (Addgene plasmid # 1764; RRID:Addgene_1764 ; ref. ). .. Finally, Ba/F3-ITD cells underwent retroviral infection with pBabe-Puro-Myr-Flag-AKT1 (Addgene plasmid # 15294; RRID:Addgene_15294 ; ref. ), containing myristoylated (constitutively activated) AKT (myr-AKT), or pBABE-puro empty vector control, as described previously ( ).

    Article Title: Pim kinase inhibitors increase gilteritinib cytotoxicity in FLT3-ITD acute myeloid leukemia through GSK-3β activation and c-Myc and Mcl-1 proteasomal degradation
    Article Snippet: .. Ba/F3-ITD cells also underwent retroviral infection with pBabe-Flag hMcl-1S159A plasmid (Addgene plasmid # 25372; RRID:Addgene_25372) containing Mcl-1 with a mutation changing serine to alanine at residue 159, preventing phosphorylation (27), pBabe-Flag hMcl-1 plasmid (Addgene plasmid # 25371; RRID:Addgene_25371), containing wild-type Mcl-1 (27), or pBabe-puro empty vector control (Addgene plasmid # 1764; RRID:Addgene_1764; ref. 24). .. Finally, Ba/F3-ITD cells underwent retroviral infection with pBabe-PuroMyr-Flag-AKT1 (Addgene plasmid # 15294; RRID:Addgene_15294; ref. 28), containing myristoylated (constitutively activated) AKT (myr-AKT), or pBABE-puro empty vector control, as described previously (24).

    Phospho-proteomics:

    Article Title: Pim Kinase Inhibitors Increase Gilteritinib Cytotoxicity in FLT3-ITD Acute Myeloid Leukemia Through GSK-3β Activation and c-Myc and Mcl-1 Proteasomal Degradation
    Article Snippet: .. Ba/F3-ITD cells also underwent retroviral infection with pBabe-Flag hMcl-1-S159A plasmid (Addgene plasmid # 25372; RRID:Addgene_25372 ) containing Mcl-1 with a mutation changing serine to alanine at residue 159, preventing phosphorylation , pBabe-Flag hMcl-1 plasmid (Addgene plasmid # 25371; RRID:Addgene_25371 ), containing wild-type Mcl-1 , or pBabe -puro empty vector control (Addgene plasmid # 1764; RRID:Addgene_1764 ; ref. ). .. Finally, Ba/F3-ITD cells underwent retroviral infection with pBabe-Puro-Myr-Flag-AKT1 (Addgene plasmid # 15294; RRID:Addgene_15294 ; ref. ), containing myristoylated (constitutively activated) AKT (myr-AKT), or pBABE-puro empty vector control, as described previously ( ).

    Article Title: Pim kinase inhibitors increase gilteritinib cytotoxicity in FLT3-ITD acute myeloid leukemia through GSK-3β activation and c-Myc and Mcl-1 proteasomal degradation
    Article Snippet: .. Ba/F3-ITD cells also underwent retroviral infection with pBabe-Flag hMcl-1S159A plasmid (Addgene plasmid # 25372; RRID:Addgene_25372) containing Mcl-1 with a mutation changing serine to alanine at residue 159, preventing phosphorylation (27), pBabe-Flag hMcl-1 plasmid (Addgene plasmid # 25371; RRID:Addgene_25371), containing wild-type Mcl-1 (27), or pBabe-puro empty vector control (Addgene plasmid # 1764; RRID:Addgene_1764; ref. 24). .. Finally, Ba/F3-ITD cells underwent retroviral infection with pBabe-PuroMyr-Flag-AKT1 (Addgene plasmid # 15294; RRID:Addgene_15294; ref. 28), containing myristoylated (constitutively activated) AKT (myr-AKT), or pBABE-puro empty vector control, as described previously (24).

    Over Expression:

    Article Title: Pim kinase inhibitor co-treatment decreases alternative non-homologous end-joining DNA repair and genomic instability induced by topoisomerase 2 inhibitors in cells with FLT3 internal tandem duplication
    Article Snippet: Ba/F3-ITD cells were infected with pMSCVpuro-Flag-cMyc-T58A retroviral plasmid (Addgene plasmid #20076; https://www.addgene.org/20076 ) [ ]) from Addgene (Cambridge, MA, USA), containing c-Myc with a point mutation at position 58, changing threonine to alanine and thereby inhibiting phosphorylation, or pMSCVpuro empty vector control (Takara Bio USA, Mountain View, CA, USA). .. To induce c-Myc overexpression, Ba/F3-ITD cells were infected with the c-Myc expression vector pBABEpuro-myc-ER (estrogen receptor) (plasmid #19128; https://www.addgene.org/19128 ) [ ] or pBABE-puro empty vector control from Addgene. ..



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    Addgene inc pbabe puro empty vector control
    Pbabe Puro Empty Vector Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbabe+puro+empty+vector+control/pBABE-puro+(Plasmid+%231764)/10__1158_slash_2767___9764__crc___23___0379-57-43-47
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